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KMID : 0377619690170060491
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Korean Jungang Medical Journal
1969 Volume.17 No. 6 p.491 ~ p.502
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Immunologic Determination of Human Chorionic Gonadotropin in Urine of Puerperas and Patients of Abnormal Pregnancies and Choriocarcinoma
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Abstract
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For the immunologic determination of H.G.G. in urine of puerperas and patients of abnormal pregnancies and choriocarcinoma, basic investigations and semi-quantitative assay has been performed using hemagglutination inhibition reaction described by Wide & Gemzell, and the following results are obtained.
1. Rabbits were immunized with relatively pure H.C.G. (5,427 iu/mg) and relatively high titer of antiserum was obtained through the effect of additional booster injections of antigen and complete Freund´s adjuvant.
2. For the direct identification of antibody to H.C.G. in the obtained antiserum, neutralization test of biological activity of H.C.G. on the weight of immature rat ovary was applied and 0.1ml of the obtained antiserum was found to neutralize the increasing effect of 200 in of H.C.G. on the ovarian weight of immature rat.
3. Ouchterlony method used in this study for the analysis of H.C.G. showed only one clear precipitin line between antigen and antiserum, indicating that the used H.C.G. is relatively pure.
4. The sensitivity of hemagglutination inhibition reaction was very high. The minium amount
of H.C.G. detected in this work was 0.2 iu/ml.
5. The sensitivity level of H.I.R. in this study was adjusted to about 600-800 iu/L of H.C.G.
6. Following term delivery through vaginal or abdominal route, urinary H.C.G. couldn´t be
detected by this method after post partum 3-7 days. After interruption of early pregnancy,
H.C.G. could be detected in urine no longer than 6-9 days postoperatively.
7. In general, significantly low urinary excretion of H.C.G. was found in cases of ectopic pregnancy, missed abotion and intrauterine fetal death.
8. In severe toxemia, urinary excretion of H.C.G. was slightly higher than that of uncomplicated pregnancy.
9. In hydatidiform mole, the excretion of H.C.G. was significantly higher, than that of normal pregnancy at the same duration. After removal of a benign mole, H.C.G. could usually be detected in the urine for 2 to 3 weeks.
10. Thee results of immunologic H.C.G. assay in 7 cases of choriocarcinoma were discussed.
11. H.I.R. was found to be a convenient and useful method in the clinical management of various abnormal conditions of pregnancy and trophoblastic tumors.
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KEYWORD
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